Waters / Protein-Pak
Protein-Pak
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The Protein-Pak™ product range, manufactured by Waters™, consists of various HPLC columns for ion-exchange and hydrophobic interaction chromatography. They are suitable for the analysis of diverse biological samples containing recombinant proteins, monoclonal antibodies, and other biological compounds.
Protein-Pak™ Hi Res Ion-Exchange (IEX) columns are based on nonporous, hydrophilic polymethacrylate particles bearing functional groups for ion-exchange chromatography. The pH-tolerant phases exhibit high protein loading capacities and show consistent batch-to-batch performance.
Protein-Pak® Hi Res HIC is a column especially for Hydrophobic Interaction Chromatography (HIC). The phases consist of nonporous, polymethacrylate based particles (2.5 μm) functionalized with a butyl-ligand coating. These particles are well-suited to characterizing proteins and biotherapeutics, including monoclonal antibodies (mAb) and antibody drug conjugates (ADC).
| Phase-Name | Modification | Particle size | Pore size | pH-Range | Tmax | Protrein Binding Capacity |
| Protein-Pak® Hi Res Q | Quaternary Ammonium | 5 µm | Non-porous | 3.0-10.0 | 60 °C | ~58 mg BSA/column |
| Protein-Pak® Hi Res CM | Carboxymethyl | 7 µm | Non-porous | 3.0-10.0 | 60 °C | ~33 mg Lysozyme/column |
| Protein-Pak® Hi Res SP | Sulfopropyl | 7 µm | Non-porous | 3.0-10.0 | 60 °C | ~25 mg Lysozyme/column |
| Protein-Pak® Hi Res HIC | Butyl | 2.5 µm | Non-porous | 2.0-12.0 | 60 °C | N/A |