Waters / Acquity

Acquity

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Acquity is a brand name for UPLC^® columns from the US manufacturer Waters. The spherical, sub-2 μm silica particles are available with various modifications and particle technologies (BEH, CSH, HSS), providing high efficiencies along with a wide selectivity space. Rugged base-particle technologies provide very high column stability and ultimate flexibility for high-throughput method development.

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Technical Data

Acquity UPLC® CSH Columns

Phase-Name	Modification	Particle size	Pore size	End-capping	C-Content	USP	Surface	pH-Range	Tmax
CSH C₁₈	C₁₈	1.7 µm	130 Å	Proprietary	15%	L1	185 m²/g	1.0-11.0	80 °C^a 45 °C^b
CSH Phenyl-Hexyl	Phenyl-Hexyl	1.7 µm	130 Å	Proprietary	14%	L11	185 m²/g	1.0-11.0	80 °C^a 45 °C^b
CSH Fluoro-Phenyl	Pentafluorophenyl	1.7 µm	300 Å	-	10%	L43	185 m²/g	1.0-8.0	60 °C^a 45 °C^b

Acquity UPLC® BEH Columns

Phase-Name	Modification	Particle size	Pore size	End-capping	C-Content	USP	Surface	pH-Range	Tmax
BEH C₁₈	C₁₈	1.7 µm	130 Å	Proprietary	15%	L1	185 m²/g	1.0-12.0	80 °C^a 60 °C^b
BEH Shield RP18	Polar embedded Alkyl	1.7 µm	130 Å	TMS	17%	L1	185 m²/g	2.0-11.0	50 °C^a 45 °C^b
BEH C₈	C₈	1.7 µm	130 Å	Proprietary	13%	L7	185 m²/g	1.0-12.0	60 °C^a 60 °C^b
Phenyl	Phenyl	1.7 µm	130 Å	Proprietary	15%	L11	185 m²/g	1.0-12.0	80 °C^a 60 °C^b
HILIC	-	1.7 µm	130 Å	-	N/A	L3	185 m²/g	1.0-9.0	45 °C^a 45 °C^b
Amide	Amide	1.7 µm	130 Å	-	12%	L68	185 m²/g	2.0-11.0	90 °C^a 90 °C^b

Acquity UPLC® HSS Columns

Phase-Name	Modification	Particle size	Pore size	End-capping	C-Content	USP	Surface	pH-Range	Tmax
HSS C₁₈	C₁₈	1.8 µm	100 Å	Proprietary	15%	L1	230 m²/g	1.0-8.0	45 °C^a 45 °C^b
HSS C₁₈ SB	C₁₈	1.8 µm	100 Å	-	8%	L1	230 m²/g	2.0-8.0	45 °C^a 45 °C^b
HSS T3	C₈	1.8 µm	100 Å	Proprietary	11%	L1	230 m²/g	2.0-8.0	45 °C^a 45 °C^b
HSS CN	Cyano	1.8 µm	100 Å	-	5%	L10	230 m²/g	2.0-8.0	45 °C^a 45 °C^b
HSS PFP	Pentafluorophenyl	1.8 µm	100 Å	-	7%	L43	230 m²/g	2.0-8.0	45 °C^a 45 °C^b

a Low pH; b High pH

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