Primesep® HPLC columns, manufactured by SIELC, are different silica-based phases for mixed-mode separations.
Acidic phases
Basic phases
Phases with the ability to form complexes
Phenyl phases
Columns with negatively charged functional groups due to the embedded anionic ion-pairing reagent.
Primesep A - Strongest acidic phase
Primesep 100 - Strong acidic phase
Primesep 200 - Medium acidic phase
Choose the correct column by their functional group acidity to match the pKa value of your basic compounds.
Columns with positively charged functional groups.
Primesep B - Strong basic column for use with TFA, phosphoric or perchloric acid solutions in a mobile phase.
Primesep B2 - Weak basic column for ammonium acetate buffered solution.
Alkyl phases with built-in functional groups that are able to form weak complexes with amino groups or metal ions.
Primesep C - Combined reverse-phase and complex formation properties for interaction with amines, sulfonium, phosphonium and metal ions.
Three interactions within one column:
Primesep P - Choose for separation of structural isomers of aromatic compounds.