SIELC / Primesep

Primesep

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Primesep® HPLC columns, manufactured by SIELC, are different silica-based phases for mixed-mode separations.


Acidic phases

  • Columns with negatively charged functional groups due to the embedded anionic ion-pairing reagent


Basic phases

  • Columns with positively charged functional groups

 

Phases with the ability to form complexes

  • Alkyl phases with built-in functional groups that are able to form weak complexes with amino groups or metal ions


Phenyl phases

  • Three interactions within one column
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Technical Data

Acidic phases

Columns with negatively charged functional groups due to the embedded anionic ion-pairing reagent.
 

Primesep A - Strongest acidic phase
Primesep 100 - Strong acidic phase
Primesep 200 - Medium acidic phase
 

Choose the correct column by their functional group acidity to match the pKa value of your basic compounds.

Basic phases

Columns with positively charged functional groups.
 

Primesep B - Strong basic column for use with TFA, phosphoric or perchloric acid solutions in a mobile phase.
Primesep B2 - Weak basic column for ammonium acetate buffered solution.

Phases with the ability to form complexes

Alkyl phases with built-in functional groups that are able to form weak complexes with amino groups or metal ions.
 

Primesep C - Combined reverse-phase and complex formation properties for interaction with amines, sulfonium, phosphonium and metal ions.

Phenyl phases

Three interactions within one column:

  • Reversed Phase
  • π-π
  • Strong-Cation Exchange
     

Primesep P - Choose for separation of structural isomers of aromatic compounds.

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