Sepax / SRT-C, Zenix-C, Unix-C

SRT-C, Zenix-C, Unix-C

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SRT-CZenix-C and Unix-C columns are silica based columns. Like the SRT, Zenix and Unix phases, the SRT-C, Zenix-C and Unix-C particles are coated with an uniform, hydrophilic and neutral film. However, this monolayer is a lay-down layer instead of a stand-up monolayer.

This is ideal for the separation of all biological samples types including challenging hydrophobic samples like antibody drug conjugates (ADCs), hydrophobic mAb and its fragments, bispecific mAbs (BsMAbs), PEGylated proteins, glycoproteins, fusion proteins, membrane proteins and oligonucleotides.

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Technical Data

SRT-C

Phase

Particle size

Pore size

Molecular range

SRT-C SEC-100

5, 10 μm

100 Å

100 - 100,000 (100 - 30,000 for 10 μm particle) Da

SRT-C SEC-150

5 μm

150 Å

500 - 150,000 Da

SRT-C SEC-300

5, 10 μm

300 Å

5,000 - 1,250,000 Da

SRT-C SEC-500

5, 10 μm

500 Å

15,000 - 5,000,000 Da

SRT-C SEC-1000

5, 10 μm

1000 Å

50,000 - 7,500,000 Da

SRT-C SEC-2000

5 μm

2000 Å

>10,000,000 Da

Zenix-C

Phase

Particle size

Pore size

Molecular range

Zenix-C SEC-80

3 μm

80 Å

100 - 50,000 Da

Zenix-C SEC-100

3 μm

100 Å

100 - 100,000 Da

Zenix-C SEC-150

3 μm

150 Å

500 - 150,000 Da

Zenix-C SEC-300

3 μm

300 Å

5,000 - 1,250,000 Da

Unix-C

Phase

Particle size

Pore size

Molecular range

Unix-C SEC-300

1.8 μm

300 Å

5,000 - 1,250,000 Da

Applications

Analysis of MAb on SRT®-C 300

Determination of MAb with SRT-C300.

Test Conditions:

Column: SRT®-C 300 (5 μm, 300 Å, 7.8x300 mm)

Mobile Phase: 150 mM sodium phosphate buffer, pH 7

Temperature: ambient (~23° C)

Flow rate: 1.0 mL/min

Detection: UV 214 nm

Concentration: 5 mg/mL

Injection volume: 2.5 μL

Sample: MAb

MAb321 Separation on Unix-C SEC-300

Seperation of MAb321 on Unix-C SEC-300.

Test Conditions:

Column: Unix-C SEC-300 (1.8 μm, 300 Å, 4.6 x 300 mm)

Mobile Phase: 150 mM phosphate buffer

Flow rate: 0.3 mL/min

System: UHPLC

Detection: UV 280 nm

Column temperature: ambient

Injection volume: 2μL

Sample: 5mg/ml MAb321

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