Sepax / Proteomix

Proteomix

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Proteomix Ion-exchange columns are especially designed for the separation of proteins, oligonucleotides, carbohydrates and peptides. The particles are made of highly cross-linked, non-porous polystyrene divinylbenzene. The surface of the particles is grafted with a hydrophilic, neutral polymer layer and ion-exchange groups are bound on this layer.
 

Proteomix HIC columns are specially designed for the separation of proteins, monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), oligonucleotides and peptides via the hydrophobic interaction chromatography (HIC) mechanism. The resin is made of non-porous polystyrene divinylbenzene (PS/DVB) and is modified with alkyl groups.
 

Proteomix RP columns are made of porous polystyrene divinylbenzene (PS/DVB). There are phenyl and substituted phenyl groups on the surface which enable hydrophobic interaction.

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Technical Data

Proteomix ion exchange

Phase

Particle size

pH range

Proteomix SCX-NP

1.7, 3, 5, 10 μm

2.0-12

Proteomix WCX-NP

1.7, 3, 5, 10 μm

2.0-12

Proteomix SAX-NP

1.7, 3, 5, 10 μm

2.0-12

Proteomix WAX-NP

1.7, 3, 5, 10 μm

2.0-12

Proteomix HIC

Phase

Modification

Particle size

pH range

Tmax

Proteomix HIC Etdyl

C2

1.7, 5, 10 μm

2.0-12

80 °C

Proteomix HIC Propyl

C3

5, 10 μm

2.0-12

80 °C

Proteomix HIC Butyl

C4

1.7, 5, 10 μm

2.0-12

80 °C

Proteomix HIC Phenyl

Ph

5, 10 μm

2.0-12

80 °C

Proteomix RP

Phase

Modification

Particle size

Pore size

pH range

Tmax

Proteomix RP

Ph, substituted Ph

5, 10 μm

300, 500, 1000 Å

1.0-14

80 °C

Applications

mAb321/Erbitux/Rituximab at 80 ℃

Determination of Ertibux, Rituximab and mAb 321 with Proteomix ® RP -1000.

Test Conditions:

Column: Proteomix ® RP -1000, 5 μm, 1000 Å, 2.1 x 150 mm

Mobile Phase A: H2O + 0.1% TFA

Mobile Phase B: ACN + 0.1% TFA

Flow rate: 0.25 mL/min

Gradient: 23- 70% B in 30 min

Detection: UV 214 nm

Column temperature: 80°C

Injection volume: 2.5 μL

Sample: monoclonal antibody, 1mg/mL diluted with 0.1% TFA

RituximAb separation - NP5 and 1.7 µm

Abtrennung von RituximAb with NP5 und 1.7 µm.

Test Conditions:

Columns: Proteomix® HIC Butyl NP1.7 μm (1.7 μm, 4.6 x 100 mm)

              Proteomix® HIC Butyl-NP5 μm (5 μm, 4.6 x 100 mm)

Mobile Phase A: 100 mM Natriumphosphatpuffer, 2 M Ammoniumsulfat, pH 7.0

Mobile Phase B: 100 mM Natriumphosphatpuffer, pH 7.0

Flow rate: 0.8 mL/min

Detection: UV 214 nm

Column temperature: 25°C

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