Sepax / Opalshell-C18

Opalshell-C18

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Opalshell™-C18 are the latest columns from Sepax based on the solid-core technology. As the stationary phase, silica particles are used which consist of a solid non-porous core surrounded by a porous silica layer. The advantages of this technology are high separation efficiencies, which are in the range of fully porous sub-2 μm particles but have a low back pressure in the region of the fully porous material of the same size.

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Technical Data

Phase

Modification

Particle size

Pore size

Surface

pH range

Tmax

Opalshell-C18

C18

2.6 µm

90 Å

150 m²/g

1.5-10

60 °C

The spherical silica particles of the Opalshell ™ -C18 columns have a very small particle size distribution and have a mean size of 2.6 μm - composed of a 1.6 μm non-porous core and an overlying 0.5 μm layer of a porous C18- modified silica material.

What are the benefits of the Opalshell ™-C18 columns?

  • Great selectivity and peak symmetry for separations of acidic, neutral and basic organic compounds
  • Wide pH-range: 1,5 to 10
  • Fast and efficient analysis
  • High stability and long column lifetime
  • Compatible with both UHPLC and HPLC for easy method transfer between systems

Applications

Application example:

Column: Opalshell™-C18 (2.6 µm, 90 Å, 4.6 x 50 mm)
Mobile Phase: A: H2O; B: ACN; C: MeOH (v/v)
Injection volume: 10 µL
Flow rate: 1.0 mL/min
Pressure: 120 bar
Detector: UV 241 nm
Temperature: Ambient
Sample: Progesterone sample mixture (0.5 mg/mL)

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