The original developer and manufacturer of Vydac-TP‒, Vydac-MS‒, Vydac Everest™‒ and Vydac Denali™ columns was Grace Vydac. Since May 2016, the new manufacturer is the britain specialist Hichrom Ltd.

Vydac TP was the first range of wide pore media phases developed in the Vydac range and became the industry standard for peptide and protein separations. The large pores of the 300 Å TP silica give polypeptide molecules complete access to the interior of the silica pores. Other features of Vydac TP Phases are a long column lifetime, a very low phase bleeding and the availability in analytical and preparative dimensions.

Vydac MS columns are a later development from Grace for the reversed-phase HPLC separation of biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns unique selectivity. A variety of reversed-phases makes this product line suitable for the analysis of small peptides to large intact, undenatured proteins. Vydac MS phases show excellent peak shape with little or no TFA and high protein recovery rates. 

Everest™ columns have unique selectivity and sensitivity, which are the result of bonding technology that improves C18 surface coverage and deactivates residual silanols. The high carbon coverage of Everest™ columns increases column life and reduces the amount of TFA required to shield the silica.

Vydac Denali™ (238DE) is a 120Å C18 bonded phase with high carbon coverage, suitable for the analysis of both acidic and basic analytes.