Advanced Materials Technology - AMT / HALO OLIGO C18

HALO OLIGO C18

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HALO OLIGO C18 is a new phase from Advanced Materials Technology (AMT). This phase is based on the well-known fused-core technology and has an orragno-silane modified surface for stability at high pH values. Furthermore, this phase is packed in passivated column hardware to prevent adsorption of the analytes on the stainless steel surface. With a pore size of 120Å, HALO OLIGO C18 is suitable for oligonucleotides with up to 60 bases. Due to its high pH and temperature stability, it is ideally suited to the conditions of oligonucleotide analyses.

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Technical Data

Phase

Modification

Particle size

Pore size

Endcapping

C-content

USP

Surface area

pH range

Tmax

HALO OLIGO C18

Organo modified surface

C18

2.7 µm

120 Å

+

5.6 %C

L1

75 m²/g

2.0-9.0

90°Ca

85°Cb

a low pH value; b high pH value

What characterises the HALO OLIGO C18?

High lot-to-lot reproducibility

  • Excellent solder-to-solder reproducibility at high pH values and temperatures

Peak Identities

1. 10 mer  2. 15 mer  3. 20 mer  4. 25 mer  5. 30 mer  6. 40 mer  7. 50 mer  8. 60 mer

Column

HALO 120Å OLIGO C18, 2.7µm, 50x2.1mm (P2A62-402)

Stability at high pH value and temperature

  • High stability at pH 10 and 60°C

High stability for over 500 injections (20,000 column volumes) with a change in retention of less than 1% at pH 10 and 60°C.

Applications

When will you benefit from the HALO OLIGO C18?

Oligonucleotide separation with good peak shape

  • Separation of oligonucleotides up to 60 bases (60 mer) with good peak shape

Peak Identities

1. 10 mer  2. 15 mer  3. 20 mer  4. 25 mer  5. 30 mer  6. 40 mer  7. 50 mer  8. 60 mer  * Tris HCl/EDTA

Column

AMT: HALO 120Å OLIGO C18, 2.7µm, 50x2.1mm

Competitor: OLIGO 130Å C18, 1.7µm, 50x2.1mm

Oligonucleotide separation under MS compatible conditions

  • Resolution of medium-length synthetic oligonucleotides under MS-compatible conditions

Peak Identities

Peak Nr.

Peak Identities

ssDNA Sequence

Retention Time

Temperature

1

T7 Terminator (19 mer)

GCT AGT TAT TGC TCA GCG G

4.49 min

60.3 °C

2

16s rRNA (20 mer)

AGA GTT TGA TCC TGG CTC AG

4.68 min

61.3 °C

3

T7 Promoter (20 mer)

TAA TAC GAC TCA CTA TAG GG

4.74 min

55.2 °C

4

16s rRNA (21 mer)

ACG GCT ACC TTG TTA CGA CTT

4.84 min

63.5 °C

5

dT (15 mer)

TTT TTT TTT TTT TTT

4.99 min

38.6 °C

6

dT (16 mer)

TTT TTT TTT TTT TTT T

5.12 min

40.9 °C

Column

HALO 120Å OLIGO C18, 2.7µm, 50x2.1mm

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Brochures

Applications

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