HALO HPLC Columns | MZ-Analysentechnik GmbH

Technical Data

HALO® 2 HALO® 2.7 and HALO® 5 (U)HPLC columns:

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
HALO^® C30	C₃₀	2.7 µm	160 Å	+	4.5%	L62	90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® C18	C₁₈	2 µm 2.7 µm 5 µm	90 Å	+	7.5% 7.7% 5.4%	L1	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO LPH-C18	C₁₈	2 µm 2.7 µm	90 Å	-	6.5%	L1	120m²/g 135m²/g	1.0-8.0	90°C^a 40°C^b
HALO^® AQ-C18	C₁₈	2 µm 2.7 µm 5 µm	90 Å	+	6.5% 6.7% 5.6%	L1	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® PCS-C18	positively charged surface C₁₈	2.7 µm	90 Å	+	7.5%	L1	125 m²/g	2.0-7.0	60°C^a 40°C^b
HALO^® C8	C₈	2 µm 2.7 µm 5 µm	90 Å	+	4.8% 5.4% 3.7%	L7	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® Phenyl-Hexyl	Phenylhexyl	2 µm 2.7 µm 5 µm	90 Å	+	6.3% 7.1% 5.2%	L11	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® Biphenyl	Biphenyl	2.7 µm	90 Å	+	7.0 %	L11	135m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® PCS Phenyl-Hexyl	positively charged surface Phenyl-Hexyl	2.7 µm	90 Å	+	6.1%	L11	125 m²/g	2.0-7.0	60°C^a 40°C^b
HALO^® ES-CN	Diisopropylcyanopropyl	2 µm 2.7 µm 5 µm	90 Å	+	3.5% 3.5% 2.5%	L10	120m²/g 135m²/g 90m²/g	1.0-8.0	80°C^a 40°C^b
HALO^® PFP	Pentafluorophenylpropyl	2 µm 2.7 µm 5 µm	90 Å	+	5.3% 5.5% 3.9%	L43	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® RP-Amide	C₁₆ Amide	2 µm 2.7 µm 5 µm	90 Å	+	7.3% 8.2% 5.5%	L60	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® HILIC	-	2 µm 2.7 µm 5 µm	90 Å	N/A	-	L3	120m²/g 135m²/g 90m²/g	1.0-8.0	60°C^a 40°C^b
HALO^® Penta-HILIC	Penta-hydroxy ligand	2 µm 2.7 µm 5 µm	90 Å	-	2.8% 3.2% 2.1%	L95	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b

a low pH; b high pH

HALO® Bioclass Columns

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
HALO^® Protein ES-C18	C₁₈	2.7 µm 3.4 µm	1000 Å 400 Å	+	1.4% 1.0%	L1	15 m²/g	1.0-8.0	90°C^a 40°C^b
HALO^® Protein C4	C₄	2.7 µm 3.4 µm	1000 Å 400 Å	+	0.6% 0.4%	L26	15 m²/g	2.0-9.0	90°C^a 40°C^b
HALO^® Protein Diphenyl	Diphenylmethyl	2.7 µm 3.4 µm	1000 Å 400 Å	+	N/A 1.0%	L11	15 m²/g	2.0-9.0	90°C^a 40°C^b
HALO^® Peptide ES-C18	C₁₈	2 µm 2.7 µm 5 µm	160 Å	-	4.0% 4.6% 4.0%	L1	65 m²/g 90 m²/g 60 m²/g	1.0-8.0	90°C^a 40°C^b
HALO^® PCS-C18	positively charged surface C₁₈	2.7 µm	160 Å	+	5.1%	L1	125 m²/g	2.0-7.0	60°C^a 40°C^b
HALO^® Peptide ES-CN	Diisopropylcyanopropyl	2.7 µm 5 µm	160 Å	+	2.2% 1.5%	L10	90 m²/g 60 m²/g	1.0-8.0	90°C^a 40°^Cb
HALO^® Peptides Phenyl-hexyl	Phenylhexyl	2.7 µm	160 Å	+	4.7%	L11	90 m²/g	2.0-9.0	90°C^a 40°C^b
HALO^® Glycan	Penta-hydroxy ligand	2.7 µm	160 Å	-	3.2%	N/A	135 m²/g	2.0-9.0	65°C^a 40°C^b

a low pH; b high pH

HALO® Enviroclass columns:

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
HALO^® PAH	C₁₈	2.7 µm	90 Å	-	9.9%	N/A	135m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® PFAS	C₄	2.7 µm	90 Å	+	N/A	N/A	135m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® PFAS Delay	Proprietary	2.7 µm	90 Å	+	N/A	N/A	90m²/g	2.0-9.0	60°C^a 40°C^b

a low pH; b high pH

Applications

HALO® Biphenyl

The HALO® Biphenyl is ready for the challenge of complex samples. Both polar and non-polar pesticides are well resolved with this commonly used test for cannabis.

ADVANTAGES OF THE NEW HALO® BIPHENYL

Improved retention, high sensitivity and higher efficiency without sacrificing robustness or reproducibility
Increased selectivity for challenging polar compounds
High efficiency leading to sharply resolved peaks with excellent peak shapes

Peak Identities

1 Daminozide 2 Flonicamid 3 Thiamethoxam 4 Imidacloprid 5 Paclobutrazol 6 Fenhexamid
7 Myclobutanil 8 Bifenazate 9 Dimethomorph Isomer 1 10 Pirotetramat 11 Dimethomorph Isomer 2
12 Spinosad A 13 Spinosad D 14 Trifloxystrobin 15 Spinetoram 16 Pyrethrin II 17 Piperonyl butoxide
18 Pyrethrin I 19 Etoxazole 20 Abamectin A 21 Cypermethrin 22 Bifenthrin 23 Acequinocyl

Isomer match

Tocopherols, a form of vitamin E known for its antioxidant properties, contain many isomers that can be easily separated in the baseline on the HALO® C30 compared to a C18 phase due to the phase's shape selectivity.