AMT - Advanced Materials Technology / HALO LPH-C18

HALO LPH-C18

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The new HALO® LPH-C18 column from Advanced Material Technology (AMT) is a sterically protected C18 phase based on a core-shell material for low pH values. This steric protection reduces the acidic hydrolysis, making the columns suitable for pH up to 1. The particles are available in 2 and 2.7 μm and in lengths up to 150 mm. Moreover, the 2.7 μm particles are also available in the new dimension of 1.5 mm ID.

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Technical Data

Phase

Modification

Particle size / μm

Pore size / Å

Carbon content / %C

Surface area / m²/g

Endcapped

pH

USP Code

HALO-LPH C18

Diisobutyloctadecylsilane

2
2.7

90

6.5

120
135

-

1a-8b

L1

(a) Tmax: 90 °C; (b) Tmax: 40 °C
 

Applications

What distinguishes the HALO® LPH-C18?

  • highly stable at low pH values at high temperatures
  • high lot-to-lot reproducibility

HALO 90 Å LPH-C18, 2.7 µm 2.1x50 mm

A separation of parabens is performed on a HALO 90 Å LPH-C18 column under low pH (pH 1) and high temperature conditions compared to a standard C18 SPP column. Due to the sterically protected ligand, the LPH-C18 column can withstand these harsh conditions over a 55 hour test.

TEST CONDITIONS:

Column: HALO 90 Å LPH-C18, 2.7 µm 2.1x50 mm

Part Number: 92822-416

Mobile Phase A: Water, 1% TFA (pH: 1)

Mobile Phase B: Acetonitrile

Gradient: Time %B
  0.0 20
  7.50 20
  7.51 5
  45.00 5
  47.00 100
  51.00 100
  51.01 20
  60.00 20

HALO 90 Å LPH-C18 2.7µm 4.6 x 50 mm

Excellent lot-to-lot reproducibility is observed with a mixture of neutral compounds.

TEST CONDITIONS:

Column: HALO 90 Å LPH-C18 2.7µm 4.6 x 50mm

Mobile Phase A: Water

Mobile Phase B: Acetonitrile

Isocratic: 60/40 Acetonitrile/Water

Wavelength: 254 nm

Injection: 2.0 µL (uracil, phenol, 1-Cl-4-nitrobenzene, naphthalene)

Temperature: 30 °C

Flow Cell: 1.8 mL/min.

When do you benefit from the HALO® LPH-C18?

You can benefit from the HALO® LPH-C18 in applications which require a low pH value. Especially in applications containing polyphenols, cannabinoids and pesticides.

HALO 90 Å LPH-C18, 2.7 µm 2.1 x 100 mm

Common polyphenols found in wine are separated using a HALO 90 Å LPH-C18 column using analytical standards. This stationary phase contains a sterically protected ligand which is ideal for high stability under low pH conditions.

PEAK IDENTITIES

1. Gallic Acid  2. Epigallocatechin  3. Chlorogenic Acid  4. Catechin  5. Caffeic Acid  6. Epicatechin  7. Epigallocatechin Gallate  8. p-Coumaric Acid  9. Ferulic Acid  10. o-Coumaric Acid  11. Quercitrin  12. Myricetin  13. Resveratrol  14. Morin  15. Quercetin  16. Naringenin  17. Apigenin  18. Kaempfero

HALO 90 Å LPH-C18, 2.7 µm, 4.6 x 150 mm

Common polyphenols found in wine are separated using a HALO 90 Å LPH-C18 column using analytical standards. This stationary phase contains a sterically protected ligand which is ideal for high stability under low pH conditions.

PEAK IDENTITIES

1. Cannabidivarinic acid (CBDVA)  2. Cannabidivarin (CBDV)  3. Cannabidiolic acid (CBDA)  4. Cannabigerolic acid (CBGA)  5. Cannabigerol (CBG)  6. Cannabidiol (CBD)  7. Tetrahydrocannabivarin (THCV)  8. Tetrahydrocannabivarinic acid (THCVA)  9. Cannabinol (CBN)  10. Cannabinolic acid (CBNA)  11. Exo-tetrahydrocannabinol (EXO-THC)  12. delta 9- Tetrahydrocannabinol (D9-THC)  13. delta 8- Tetrahydrocannabinol (D8-THC)  14. Cannabicycol (CBL)  15. Cannabichromene (CBC)  16. Tetrahydrocannabinolic acid A (THCA-A)  17. Cannabichromenic acid (CBCA)  18. Cannabicyclolic acid (CBLA)

HALO 90 Å LPH-C18 2 µm, 2.1 x 100 mm

Pesticides screening of crops, in this example barley being the crop of concern, is performed using LCMS with the 2 µm HALO 90 Å LPH-C18 where both speed and resolution are demonstrated.

PEAK IDENTITIES

1. Carbendazim  2. Dicrotophos  3. Azamethiphos  4. Pyrimethani  5. Carbofuran  6. Dodemorph  7. Atrazine  8. Diuron  9. Iprovalicarb  10. Azoxystrobin  11. Fluopram  12. Methoxyfenozide  13. Flutolanil  14. Picoxystrobin

Conclusion: The HALO® LPH-C18 is suitable for applications at low pH values and also offers fast, efficient and rugged separations based on the core-shell technology.

As AMT's exclusive distributor in Germany, you can get the new HALO® LPH-C18 directly from us. We would be pleased to advise and support you in the selection of the suitable column. Contact our support team for a consultation and an individual quote. We are looking forward to your contact!

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