HALO Glycan HPLC Columns | MZ-Analysentechnik GmbH

The Halo Glycan Columns from Advanced Materials Technology are the newest member of the Halo Fused BioClass family. They are especially designed for bioseparations of oligosaccharides and particularly protein-linked glycans and glycopeptides by HILIC, H(ydrophilic) I(interactive) LI(quid) C(hromatography).

Chemistry:

Highly polar ligand with 5 hydroxyl groups
Ligand tethered to 2.7µm Fused Core silica via novel proprietary linkage chemistry
Fused-Core particle with 135 m2/g surface area and 90A pore size

For separations of:

Oligosaccharides
Protein-linked glycans andglycopeptides
Example application: RNGase-Released and Labeled N-Glycans (see right)

For Quality Assurance QA every lot is tested by separation of a procainamide-reducing end-labeled glycan ladder of oligosaccharides of 2-25 glucose units. The peaks of 5 and 10 glucose units have to meet strict specifications of retention and peak widths before the lot is approved for the HALO Glycan columns.

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Applications

Separation of Procainamide-Labeled Dextran Standards on HALO Glycan

A HALO Glycan column shows an efficient separation of procainamide-labeled dextran standards (Sigma-Aldrich 1:1 (w/w) of Part numbers 00268 and 00269) at 0.5 µg/µL in 70% ACN/30% water. Each lot of HALO Glycan packing is tested using this sample to assure lotto-lot reproducibility and performance.

TEST CONDITIONS

Column: 2.1 x 150 mm, HALO 2.7 μm Glycan
Part Number: 92922-705
Mobile Phase A: 50 mM Ammonium Formate, pH 4.45
Mobile Phase B: Acetonitrile
Gradient: 80-55% B in 25 min
Flow Rate: 0.6 mL/min.
Pressure: 190 bar
Temperature: 60°C
Detection: UV 300 nm
Injection Volume: 3 μL Sample
Solvent: 70/30 ACN/water
Response Time: 0.5 sec.
Data Rate: 3.3 Hz
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Nexera

Separation of PNGase-Released and Labeled N-Glycans By HILIC Using HALO Glycan Column

A fast separation of PNGase-released and procainamidelabeled N-Glycans from Ribonuclease B is accomplished with a HALO Glycan column.

PEAK IDENTITIES:

1. PAm-GlcNAc2Man5
2. PAm-GlcNAc2Man6
3. PAm-GlcNAc2Man7
4. PAm-GlcNAc2Man8
5. PAm-GlcNAc2Man9

HALO Glycan

Applications

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