Supelcosil™ is a product line of Supelco® Analytical, which is a division of Sigma-Aldrich (a subsidiary of Merck since 2015). All Supelcosil columns are based on spherical silica gel, which is available in various modifications. The product range extends from unmodified normal to medium polar and reversed phases. In addition, the Supelcosil SAX1 and the Supelcosil LC-SCX are also available as anion and cation exchange columns.
With the Supelcosil Hisep, there is also a mixed-mode column that has a protected hydrophobic phase. The silica gel is encased in a thin polymer layer with hydrophobic regions in a hydrophilic network. This allows small analytes to be retarded and proteins to be excluded.
Supelcosil LC-18-DB and Supelcosil LC-8-DB are characterised by special deactivation, which means that the columns have shorter retention times and better peak shapes for organic bases.
Supelcosil ABZ+Plus and Supelcosil ABZ are phases that are used for basic, acidic, polar neutral and non-polar components thanks to a unique deactivation technology. These columns are complemented by Supelcosil Suplex pKb-100, which has the same modification but no end-capping. This gives it better performance with strongly basic components.
| Phase | Modification | Description | USP Code |
| Supelcosil LC-18 | C18 | hydrophobic C18 phase with high retention and good peak shapes for many compounds | L1 |
| Supelcosil LC-18-DB | C18 | Reversed-phase column with special deactivation, shows shorter retention, better peak shapes and higher efficiency for organic bases | L1 |
| Supelcosil LC-18-S | C18 | Reversed-phase column for the separation of deoxyribonucleosides and ribonucleosides | L1 |
| Supelcosil LC-18-T | C18 | Reversed-phase column with special surface treatment for the separation of nucleotides | L1 |
| Supelcosil LC-8 | C8 | Reversed phase with lower hydrophobic phase than C18 for lower retention of polar and non-polar compounds | L7 |
| Supelcosil LC-8-DB | C8 | Reversed-phase column with special deactivation, shows shorter retention, better peak shapes and higher efficiency for organic bases | L7 |
| Supelcosil LC-1 | C1 | Mixed retention mode for higher selectivity for polar groups | L13 |
| Supelcosil LC-DP | Diphenyl | Reversed-phase column with higher selectivity for aromatic groups | L11 |
| Supelcosil LC-CN | Cyanopropyl | medium polar column, which is used in HILIC, normal and reversed phase chromatography | L10 |
| Supelcosil LC-PCN | Cyanopropyl | Reversed-phase column preconditioned for the determination of tricyclic anti-depressants | L10 |
| Supelcosil LC-NH2 | Aminopropyl | medium polar column for HILIC and normal phase for the determination of mono- and disaccharides | L8 |
| Supelcosil LC-NH2-NP | Aminopropyl | Normal-phase column in which water was omitted in the preparation, resulting in stable retention (use only with non-aqueous mobile phases!) | L8 |
| Supelcosil LC-Diol | diol | Medium polar column for HILIC and normal phase for the separation of proteins by gel filtration and the separation of steroids and sterols | L20 |
| Supelcosil ABZ+Plus | Alkylamides | Reversed-phase column with high deactivation and unique selectivity for polar and charged compounds | L60 |
| Supelcosil LC-ABZ | Alkylamides | Reversed-phase column with unique deactivation technology that provides excellent performance for basic, acidic, polar neutral and non-polar compounds | L60 |
| Supelcosil Suplex pKb-100 | Alkylamides | Reversed-phase column with the same modification as Supelcosil LC-ABZ but without end-capping, resulting in better performance with strongly basic components | L68 |
| Supelcosil SAX1 | Propyltrime- thylammonium | Anion exchanger with strongly basic quaternary aminopropyl groups | L14 |
| Supelcosil LC-SCX | Propylsulfonic acid | Cations Exchanger with strongly acidic propylsulfonic acid groups | L52 |
| Supelcosil LC-PAH | C18 | Reversed-phase column especially for the analysis of polycyclic aromatic hydrocarbons | L1 |
| Supelcosil LC-Si | - | typical silica gel column for HILIC or normal phase chromatography | L3 |
| Supelcosil Hisep | Shielded hydrophobic phase | mixed-mode column with hydrophobic polymer coating and hydrophilic network, so that small analytes can penetrate the hydrophilic network and interact with hydrophobic groups, proteins are excluded and not retarded | N/A |