Tosoh Bioscience / TSKgel FcR-IIIA-NPR

TSKgel FcR-IIIA-NPR

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For the fast assessment for biologic activity of mAbs

Tosoh Bioscience GmbH introducing the new affinity column - TSKgel FcR-IIIA-NPR - based on a recombinant FcγIIIa receptor ligand. The column is designed for fast assessment for biologic activity of mAbs. 


The FcγIIIa receptor plays a key role in antibody-dependent cell mediated cytotoxicity (ADCC) by interacting with the N-glycans of the Fc domain of immunoglobulins. Hence, affinity chromatography using immobilized Fc receptor ligands can deliver valuable information about ADCC activity of therapeutic antibodies. As the interaction/affinity is influenced by N-glycans the separation profile also reflect the antibodies glycoform pattern.

This fast and efficient method can be used in many phases of development and production such as cell line screening in early R&D, biosimilar/originator comparison, upstream development and optimization, monitoring of glycoengineering, or lot-to-lot comparison in QC.

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Technical Data

Highlights of the product:
 

  • Easy and reproducible HPLC analysis based on FcγRIIIa affinity of mAbs
     
  • Unique glycoprotein elution profile of IgG allows assessment of ADCC activity
     
  • Applicable to purified samples and cell culture supernatant alike
     
  • Fast cell line screening, upstream development, lot-to-lot comparison
Description

Matrix

Ligand

Particle size

pH-Range

T-Range

Housing

Dimension

TSKgel® FcR-IIIA-NPR

Hydrophilic non-porous Polymer

Modified recombinant human FcγRIIIA

5 µm

4.0−8.0

15−25°C

PEEK

75x4.6mm

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