Tosoh Bioscience / TSKgel FcR-IIIA-NPR
TSKgel FcR-IIIA-NPR
Tosoh Bioscience GmbH introducing the new affinity column - TSKgel FcR-IIIA-NPR - based on a recombinant FcγIIIa receptor ligand. The column is designed for fast assessment for biologic activity of mAbs.
The FcγIIIa receptor plays a key role in antibody-dependent cell mediated cytotoxicity (ADCC) by interacting with the N-glycans of the Fc domain of immunoglobulins. Hence, affinity chromatography using immobilized Fc receptor ligands can deliver valuable information about ADCC activity of therapeutic antibodies. As the interaction/affinity is influenced by N-glycans the separation profile also reflect the antibodies glycoform pattern.
This fast and efficient method can be used in many phases of development and production such as cell line screening in early R&D, biosimilar/originator comparison, upstream development and optimization, monitoring of glycoengineering, or lot-to-lot comparison in QC.
Highlights of the product:
| Description | Matrix | Ligand | Particle size | pH-Range | T-Range | Housing | Dimension |
| TSKgel® FcR-IIIA-NPR | Hydrophilic non-porous Polymer | Modified recombinant human FcγRIIIA | 5 µm | 4.0−8.0 | 15−25°C | PEEK | 75x4.6mm |
Tosoh TSKgel FcR-IIIA-NPR Fast Evaluation of ADCC Activity Applications
Tosoh TSKgel FcR-IIIA-NPR Product Manual Technical-Support
Tosoh TSKgel FcR-IIIA-NPR Robustness and Dynamic Range Technical-Support
Tosoh TSKgel FcR-IIIA-NPR Characterization by Top Down MS Applications