ProPac

Thermo Scientific's ProPac™ PA1 LC columns enable the analysis and purification of hydrophilic anionic proteins and peptides. These strong anion-exchange columns provide high-resolution separations of proteins with pI values from 3 to 11. A pellicular packing ensures high-efficiency and fast mass transport.
 

ProPac™ HIC-10 are columns for high-resolution separations of proteins and peptides under non-denaturing conditions via Hydrophobic Interaction Chromatography (HIC). The stationary phase is based on 5 µm, high-purity silica particles.
 

ProPac™ IMAC-10 are special columns for "Immobilized Metal Affinity Chromatography" (IMAC). IMAC is a specialty technique used primarily for the purification of proteins that contain surface-exposed histidine residues and for enrichment of phosphopeptides. It is based on a reversible interaction between metal-binding sites on the protein with metal bound to a stationary phase. The stationary phase is composed of 10 μm, nonporous, styrene-divinylbenzene beads coated with a hydrophilic layer, to eliminate secondary hydrophobic interactions with the proteins. This hydrophilic layer has linear acrylate chains with pendant iminodiacetate (IDA) groups grafted to it. The poly(IDA) grafts are converted to metal-containing nanoparticles when the column is charged with metal. It is these nanoparticles that act as the IMAC interaction sites for individual proteins.