Sepax Polar - High-Performance HPLC Columns for Precise Analyses

Polar columns from Sepax are columns for various purposes. The series includes both polymer and silica phases. Each phase has a special modification to fulfil different requirements.

Polar MC-HIC columns are made of hydrophilic polymethacrylate functionalized with ether or butyl for hydrophobic interaction chromatography (HIC).

Polar MC-IEX columns consist of hydrophilic methacrylate with ion exchange groups for ion exchange chromatography (IEX). The exchange groups are chemically bonded to the surface, creating a dense and uniform ion exchange layer.

Polar MC-IMAC columns are metal affinity chromatography columns for the purification of recombinant proteins with a histidine (His) tag and are based on hydrophilic polymethacrylate. The hydrophilic surface contains carboxylic acid groups that chelate polyvalent metal ions.

Polar-100 are silica-based columns with poly(ethylene glycol) as the functional group.

Polar imidazole columns consist of silica particles that have an imidazole functionalisation on their surface. This N-containing ring structure is a weak base and is therefore suitable for many applications, e.g. drugs, peptides, sugars and nucleotides.

Polar pyridine columns are silica-based columns in which a pyridine is chemically bound to the surface. This pyridine with its N-containing ring structure is a weak base and can be used for many different applications such as pharmaceuticals, peptides, sugars and nucleotides.

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Technical Data

Phase	Modification	Base material	Particle size	Pore size	Surface area	pH range	Endcapped
Polar MC30-HIC Butyl	C4	Hydrophilic polymethacrylate	20 - 45 µm	800 Å	N/A	2.0 - 14	N/A
Polar MC30-HIC Ether	ether	Hydrophilic polymethacrylate	20 - 45 µm	800 Å	N/A	2.0 - 14	N/A
Polar MC30-SCX	N/A	Hydrophilic polymethacrylate	20 - 45 µm	800 Å	N/A	2.0 - 12	N/A
Polar MC60-SCX	N/A	Hydrophilic polymethacrylate	45 - 90 µm	800 Å	N/A	2.0 - 12	N/A
Polar MC30-WCX	N/A	Hydrophilic polymethacrylate	20 - 45 µm	800 Å	N/A	2.0 - 12	N/A
Polar MC60-WCX	N/A	Hydrophilic polymethacrylate	45 - 90 µm	800 Å	N/A	2.0 - 12	N/A
Polar MC30-SAX	N/A	Hydrophilic polymethacrylate	20 - 45 µm	800 Å	N/A	2.0 - 12	N/A
Polar MC60-SAX	N/A	Hydrophilic polymethacrylate	45 - 90 µm	800 Å	N/A	2.0 - 12	N/A
Polar MC30-WAX	N/A	Hydrophilic polymethacrylate	20 - 45 µm	800 Å	N/A	2.0 - 12	N/A
Polar MC60-WAX	N/A	Hydrophilic polymethacrylate	45 - 90 µm	800 Å	N/A	2.0 - 12	N/A
Polar MC30-CA Excel	Carboxylic acid	Hydrophilic polymethacrylate	20 - 45 µm	800 Å	N/A	1.0 - 13	N/A
Polar MC60-CA Excel	Carboxylic acid	Hydrophilic polymethacrylate	45 - 90 µm	800 Å	N/A	1.0 - 13	N/A
Polar MC30-Ni Excel	Carboxylic group/Ni2+	Hydrophilic polymethacrylate	20 - 45 µm	800 Å	N/A	1.0 - 13	N/A
Polar MC60-Ni Excel	Carboxylic group/Ni2+	Hydrophilic polymethacrylate	45 - 90 µm	800 Å	N/A	1.0 - 13	N/A
Polar-100	Poly(ethylene glycol), polymeric	silica	1.8, 3, 5, 10 µm	120 Å	300 m²/g	N/A	+
Polar diol	Diol, polymeric	silica	1.8, 3, 5, 10 µm	120 Å	300 m²/g	N/A	+
Polar imidazoles	Imidazoles, polymeric	silica	1.8, 3, 5, 10 µm	120 Å	300 m²/g	N/A	+
Polar pyridines	Pyridines, polymeric	silica	1.8, 3, 5, 10 µm	120 Å	300 m²/g	N/A	+
Polar silica	silica	silica	1.8, 3, 5, 10 µm	60, 100, 120, 300, 500, 1000, 2000 Å	N/A	N/A	N/A