Sepax / Antibodix
Antibodix
Antibodix columns are made of non-porous polystyrene divinylbenzene polymer (PS/DVB). These columns are specially designed for high resolution, high efficiency and high recovery separations of antibodies. The resin is grafted with a highly hydrophilic, neutral polymer. On this layer weak cation exchange groups located. This results in a high capacity ion-exchange layer. The columns also available in stainless steel or PEEK housings.
| Phase | Particle size | Pressure limit | pH range | Tmax |
| Antibodix-NP1.7 | 1.7 μm | 12,000 (827) psi (bar) | 2.0-12 | 80 °C |
| Antibodix-NP3 | 3 μm | 8,000 (551) psi (bar) | 2.0-12 | 80 °C |
| Antibodix-NP5 | 5 μm | 6,000 (413) psi (bar) | 2.0-12 | 80 °C |
| Antibodix-NP10 | 10 μm | 4,000 (275) psi (bar) | 2.0-12 | 80 °C |
Analysis of MAb with charge variance on Antibodix™ NP10.
Test Conditions:
Column: Antibodix NP10 (10 μm, 4.6x250mm, Non-porous)
Mobile Phase A: 10 mM phosphate buffer pH: 7.5
Mobile Phase B: 10 mM phosphate buffer + 100 mM NaCl
Flow rate: 0.8 mL/min
Detection: UV 214 nm
Temperature: ambient
Concentration: 5 mg/mL
Injection volume: 5 µL
Sample: Monoclonal antibody
Analysis of MAb on Antibodix™ NP3
Test Conditions:
Column: Antibodix™ WCX NP3(3 µm, 7.8x75 mm)
Mobile Phase A: 10 mM sodium phosphate buffer, pH7,5
Mobile Phase B: 10 mM sodium phosphate buffer + 0.5 M NaCl
Gradient: 3-11%B in 30 min
Flow rate: 0.8 mL/min
Detection: UV 214 nm
Temperature: 25°C
Injection volume: 10 μL
Samples: Erbitux and MAb221
Sepax Antibodix NP10 Analysis of MAb with Charge Variance
Sepax Antibodix NP10 Analysis of MAb-X22 Impact of Initial Salt
Sepax Antibodix NP10 Analysis of MAb-X22 Impact of Mobile Phase Ph
Sepax Antibodix WCX-NP3 Analysis of MAb
Sepax Antibodix WCX-NP5 Proteomix SCX-NP5 Ion Exchange Kit for MAb-separation
Sepax Antibodix HPLC-UHPLC Life Science Brochure
Sepax Antibodix User Manual
2-8 Antibody_Solution_Kit_Booklet-2-14