Porvair Assay Plates - High-Quality Plates for Precise Assays

Porvair Sciences have a range of clear polystyrene bottom, glass bottom and solid bottom assay plates that are specifically designed for absorbance, fluorescence, luminescence and scintillation applications. All Assay Plates maintaining the standard ANSI/SLAS format.

Microplate readers are designed to read from either the top or the bottom of a microplate. Top reading instruments rely on measuring reflected light above the wells. A good solid bright white plate is best for these absorbance measurements and a solid black plate for fluorescence readings. Bottom reading units illuminate the sample from above and then use detectors placed below the plate to measure the absorption or fluorescence/luminescence emission. This necessitates the use of clear-bottomed plates. The requirement is to transmit the light wavelengths of interest.

Visible wavelength range (900-350nm) measurements require only clear plastic bases, whilst readings between 220nm and 350nm will require a UV-transparent material. This can be Quartz sheet or a special polymer such as Cyclo-Olefin Co-Polymer (COP/COC). Optical glass sheet (borosilicate glass) is used where visible range detection is combined with confocal optics or whole plate imaging which requires a very clear uniformly-flat base.

Clear and opaque polystyrene assay plates are suitable for top reading applications.

Porvair's Krystal™ series includes assay plates for bottom-reading applications (although some of them can be used for top-reading applications as well). Krystal™ assay plates therefore have clear bottoms.

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Technical Data

The following types of Assay Plates are available:

Clear Solid Polystyrene Assay Plates

96-well plates for routine adsorption-, ELISA-, mixing- and storage applications
Manufactured from high quality crystal polystyrene
Different bottom shapes are available
- Flat for spectrophotometric work
- V-bottom minimizing residual liquid
- U-bottom for cell/particulate collection
Robot compatible
Working volumes from 275μl down to 10μl

Opaque Solid Polystyrene Assay Plates

96-well plates in black, white or black with white wells
384-well plates in black, white and clear
For absorbance, fluorescence, luminescence and scintillation applications
Standard ‘chimney’ wells to overcome optical crosstalk and contamination
The acclaimed composite black/white plate has been shown to be ten times more sensitive than a standard white plate for chemi-luminesence assays.

Krystal™ Black & White Assay Plates

Available as 24-well, 96-well or 384-well version
Opaque walls to prevent well-to-well crosstalk
Clear plate bottom permits direct microscopic viewing
For use with top or bottom reading instruments
96-well Krystal™ 2000 plates are optimised for luminescence and fluorescence assays

Krystal™ polystyrene clear bottom

Standard clear polystyrene bottom plates
Ultrasonically welded for perfect sealing every time
0.4mm thick (384-well)
0.75mm (24- and 96-well)

Krystal™ glass bottom plates

Polystyrene plates with borosilicate glass bottom for whole-plate CCD imaging and laser detection applications
Available as 24-well, 96-well or 384-well version
175μm glass thickness and 335nm UV cut-off
Very low autofluorescence
Biocompatible adhesive
Resistant to alcohol, DMSO and PBS

Krystal™ UV clear bottomed microplates

Ideal for laboratories measuring circular dichroism (CD)
COP- or quartz bottomed, UV-transparent well-plates
Available as 96-well or 384-well version
UV cut-off (COP) at 220nm
Applications e.g. most sensitive UV range fluorescence assays using wholeplate imaging or confocal microscopy

Components for Assay & Method Development

A- and B-plates as well as lids

MZ-Analysentechnik GmbH is official distributor for Porvair Sciences in Germany. For further technical and price information please contact us!

Assay Plates

Technical Data

The following types of Assay Plates are available:

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