Perkin-Elmer / Brownlee

Brownlee

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Brownlee is a brand name for HPLC columns manufactured by Perkin Elmer™. The phases are based on high-purity, spherical silica gel and are available in many modifications for a wide variety of applications.

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Technical Data

Brownlee Analytical: HPLC phases for routine analysis based on 110 Å silica
Brownlee Analytical DB: Fully base-deactivated 140 Å silica for analysis of small molecules as well as small peptides
Brownlee Aquapore^® und Bio: HPLC phases based on 300 Å silica for analysis of biomolecules
Brownlee Choice™: HPLC phases based on 60 Å silica with high surface area, high carbon loads and high retention
Brownlee Spheri-5^® und Spheri-10^®: Small-pore (80 Å, 5 μm), silica based sorbent for separating small molecules. Both monofunctional and polyfunctional C8 and C18 sorbents available.
Brownlee Validated: High purity HPLC phases with inherent low silanophilic activity. For the analysis of acids, bases and neutral compounds.
Brownlee SPP: Core-Shell (Fused-Core™) phasen for fast and high resolution separations of various compounds. The particle size is 2.7 µm (Core: 1.7 µm).

1. Brownlee Analytical HPLC columns

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
C18	C18	3, 5 µm	110 Å	+	13%	L1	N/A	2.5-7.5	80 °C
C8	C8	5 µm	110 Å	+	7%	L7	N/A	2.5-7.5	80 °C
phenyl	phenyl	5 µm	110 Å	+	6%	L11	N/A	2.5-7.5	80 °C
Biphenyl	Biphenyl	5 µm	110 Å	+	Proprietary	L11	N/A	2.5-7.5	80 °C
Cyano	Cyano (CN)	5 µm	110 Å	+	4%	L10	N/A	2.5-7.5	80 °C
Amino	Amino (NH2)	3, 5 µm	110 Å	-	2%	L8	N/A	2.5-7.5	80 °C
PAH	C18	4 µm	110 Å	+	Proprietary	N/A	N/A	2.5-7.5	80 °C
Silica	-	5 µm	110 Å	-	-	L3	N/A	2.5-7.5	80 °C

2. Brownlee Analytical DB (U)HPLC columns

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
C18	C18	1.9, 3, 5 µm	140 Å	+	11%	L1	N/A	2.5-8.0	80 °C
Aq C18	C18	1.9, 3, 5 µm	140 Å	Proprietary	6%	L1	N/A	2.5-8.0	80 °C
Biphenyl	Biphenyl	1.9, 3, 5 µm	140 Å	+	8%	L11	N/A	2.5-8.0	80 °C
Cyano	Cyano (CN)	1.9, 5 µm	140 Å	-	4%	L10	N/A	2.5-8.0	80 °C
PFP Propyl	Pentafluorophenyl	1.9, 3, 5 µm	140 Å	+	6%	L43	N/A	2.5-8.0	80 °C
IBD	Polar group embedded Alkyl	1.9 µm	140 Å	+	Proprietary	L68	N/A	2.5-8.0	80 °C
PAH	C18	1.9 µm	140 Å	+	Proprietary	N/A	N/A	2.5-8.0	80 °C
Silica	-	1.9 µm	140 Å	-	-	L3	N/A	2.5-8.0	80 °C

3. Brownlee Aquapore and Bio HPLC columns

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
AP OD-300 (C18)	C18	7 µm	300 Å	+	10%	L1	N/A	2.5-8.0	60 °C
AP RP-300 (C8)	C8	7 µm	300 Å	+	5%	L7	N/A	2.5-8.0	60 °C
AP AX-300^[1]	Polyetdyleneimine	7 µm	300 Å	N/A	N/A	N/A	N/A	2.0-8.0	60 °C
AP CX-300^[2]	Polyamide coating with carboxymetdyl groups	7 µm	300 Å	N/A	N/A	N/A	N/A	2.0-8.0	60 °C
Bio C18	C18	5 µm	300 Å	+	9%	L1	N/A	2.5-8.0	80 °C
Bio C8	C8	5 µm	300 Å	+	5%	L7	N/A	2.5-8.0	80 °C

[1] Weak Anion Exchanger [2] Weak Cation Exchanger

4. brownlee Choice™ HPLC columns

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
C18	C18	5 µm	60 Å	+	27%	L1	N/A	2.5-8.0	80 °C
Aq C18	C18	5 µm	60 Å	-	Proprietary	L1	N/A	2.5-8.0	80 °C
Biphenyl	Biphenyl	5 µm	60 Å	-	23%	L11	N/A	2.5-8.0	80 °C
PFP Propyl	Pentafluorophenyl	3, 5 µm	60 Å	+	17%	L43	N/A	2.5-8.0	80 °C
Basix	Phenyl Cyano	5 µm	60 Å	+	12%	L10	N/A	2.5-8.0	80 °C
Organic Acid^[1]	N/A	5 µm	60 Å	+	Proprietary	N/A	N/A	2.5-8.0	80 °C
AK^[2]	N/A	5 µm	60 Å	-	Proprietary	N/A	N/A	2.5-8.0	80 °C

[1 ] Special phase for the separation of organic acids
[2] Special phase for the separation of DNPH derivatised aldehydes and ketones

5. Brownlee Spheri-5 and Spheri-10 HPLC columns

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
Spheri-5 C18	C18	5 µm	80 Å	+	11%	L1	N/A	N/A	N/A
Spheri-5 C18 ODS (Polyfunctional)	C18	5 µm	80 Å	+	14%	L1	N/A	N/A	N/A
Spheri-10 C18	C18	10 µm	80 Å	+	11%	L1	N/A	N/A	N/A
Spheri-10 C2	C2	10 µm	80 Å	-	N/A	L16	N/A	N/A	N/A
Spheri-5 Silica	-	5 µm	80 Å	N/A	N/A	L3	N/A	N/A	N/A

6. Brownlee Validated HPLC columns

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
C18	C18	3, 5 µm	100 Å	+	20%	L1	N/A	2.5-8.0	80 °C
Aq C18	C18	3, 5 µm	100 Å	-	15%	L1	N/A	2.5-8.0	80 °C
C8	C8	5 µm	100 Å	+	12%	L7	N/A	2.5-8.0	80 °C
C4	C4	5 µm	100 Å	+	9%	L26	N/A	2.5-8.0	80 °C
C1	C1	5 µm	100 Å	-	5%	L13	N/A	2.5-8.0	80 °C
phenyl	phenyl	3, 5 µm	100 Å	+	10%	L11	N/A	2.5-8.0	80 °C
Cyano	Cyano (CN)	5 µm	100 Å	+	8%	L10	N/A	2.5-8.0	80 °C
Amino	Amino (_NH2)	3, 5 µm	100 Å	-	2%	L8	N/A	2.5-8.0	80 °C
PFP Propyl	Pentafluorophenyl	5 µm	100 Å	+	11%	L43	N/A	2.5-8.0	80 °C
IBD	Polar group embedded Alkyl	3, 5 µm	100 Å	-	12%	L68	N/A	2.5-8.0	80 °C
Carbamate^[1]	N/A	3, 5 µm	100 Å	Proprietary	Proprietary	N/A	N/A	2.5-8.0	80 °C
Quat^[2]	N/A	5 µm	100 Å	Proprietary	Proprietary	N/A	N/A	2.5-8.0	80 °C

[1] Special phase for the analysis of diquat, paraquat and other quaternary amines
[2] Special phase for the analysis of carbamates

7. Brownlee SPP (U)HPLC columns

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
C18	C18	2.7 µm	90 Å	+	N/A	N/A	N/A	2.0-9.0	60 °C
Peptides ES-C18	C18	2.7 µm	160 Å	+	N/A	N/A	N/A	1.0-8.0	90 °C
C8	C8	2.7 µm	90 Å	+	N/A	N/A	N/A	2.0-9.0	60 °C
Phenylhexyl	Phenylhexyl	2.7 µm	90 Å	+	N/A	N/A	N/A	2.0-9.0	60 °C
PFP	Pentafluorophenyl	2.7 µm	90 Å	+	N/A	N/A	N/A	2.0-8.0	60 °C
RP amides	Amide embedded Alkyl	2.7 µm	90 Å	+	N/A	N/A	N/A	2.0-9.0	60 °C
HILIC	N/A	2.7 µm	90 Å	-	N/A	N/A	N/A	2.0-8.0	60 °C

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Brochures

HPLC GC SPE
Perkin-Elmer Chromatography Consumables and Supplies Catalogue 2017-2018

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