Nacalai Tesque / Cosmosil 1.8C18-MS-II

Cosmosil 1.8C18-MS-II

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The COSMOSIL 1.8C18-MS-II columns from Nacalai Tesque are new fully porous columns for UHPLC applications. The small 1.8µm particles allow a high number of theoretical plates and thus good separations even with short columns. Due to the high hydrophobicity of the COSMOSIL 1.8C18-MS-II columns, even demanding separations of hydrophobic analytes can be separated. The columns are designed for MS detection and are therefore available in 2.1mm inner diameter and in lengths of 30, 50, 75, 100 and 150mm.

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Technical Data

Phase

Modification

Particle size

Pore size

Surface area

Max. Pressure

pH

COSMOSIL 1.8C18-MS-II

C18

1.8 µm

120 Å

340 m²/g

800 bar

2.0-10.0

Applications

What characterises the COSMOSIL 1.8C18-MS-II columns?

Durability test COSMOSIL 1.8C18-MS-II 50x2.1mm

  • Excellent service life of the pillar

Column: COSMOSIL 1.8C18-MS-II 50x2.1mm

Mobile Phase A: 0.1% TFA in Water

Mobile Phase B: 0.1% TFA in Acetonitrile

Gradient: 

Time / min

%B

0-3.00

5-90

3.00-3.01

90-5

3.01-6.00

5

Flow rate: 0.4 mL/min

Temperature: 40 °C

Detection: UV 236 nm

Sample: Amitriptyline (0.2 mg/mL)

Injection volume: 1.0 µL

Separation of structural isomers of vitamin D (COSMOSIL 1.8C18-MS-II 50x2.1mm)

  • Available particle sizes range up to 15µm; simple scaling for preparative processes

Peak Identities

1. Previtamin D3

2. Vitamin D3

3. Lumisterol

4. 7-Dehydrocholesterol (7-DHC)

When will you benefit from the COSMOSIL 1.8C18¬-MS-II?

Drugs (NSAIDs) COSMOSIL 1.8C18-MS-II 50x2.1mm

  • Strong hydrophobic properties of the phase allow the separation of challenging analytes

Peak Identities

1. Acetylsalicyclic acid  2. Loxoprofen  3. Ketoprofen  4. Naproxen  5. Phenylbutazone  6. Celecoxib  7. Diclofenac  8. Indometacin

Flavonoid analysis (COSMOSIL 1.8C18-MS-II 50x2.1mm)

  • Phase selectivity can enable difficult separations that co-elute on other columns

Peak Identities

1. Myricetin  2. 7,8-Dihydroxyflavone  3. Quercetin  4. Apigenin  5. Kaempferol  6. 7-Hydroxyflavone  7. 6-Hydroxyflavone  8. Flavone  9. Chrysin  10. 6-Methoxyflavone  11. 3-Hydroxyflavone  12. 5-Hydroxyflavone

Conclusion: With the COSMOSIL 1.8C18-MS-II phase, hydrophilic substances can be optimally separated and at the same time an alternative selectivity to other C18 columns is possible. The availability of particle sizes of up to 15µm makes the scale-up process easy to manage.

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