SeQuant^® ZIC^® HILIC phases are based on high purity, spherical silica. They carry densely bonded, truly zwitterionic sulfobetaine groups, with an outer negative charge and an inner positive charge.  
 

SeQuant^® ZIC^® cHILIC phases are also based on high purity, spherical silica. They carry densely bonded, truly zwitterionic phosphorylcholine groups, with an outer positive charge and an inner negative charge. The selectivity of ZIC^®-cHILIC is complementary to ZIC^®-HILIC, due to their differently oriented zwitterionic functional groups. The outermost, more accessible, charged moiety, tend to dominate the interaction with solutes. Positively charged compounds thus typically have lower retention on ZIC^®-cHILIC whereas negatively charged compounds have higher retention. Neutral compounds tend to experience more similar retention on the two zwitterionic columns.
 

SeQuant^® ZIC^®pHILIC phases are polymer-based and carry densely bonded, truly zwitterionic sulfobetaine groups. They can be used in an extended pH range and therefore offer additional flexibility during method development.