Merck / Chromolith

Chromolith

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Merck´s Chromolith® HPLC columns are based on monolithic silica. In contrast to conventional particle-packed columns, Chromolith® phases are produced from a continuous piece of porous silica using a sol-gel process. The stationary phase possess a defined bimodal pore structure with macro- and mesopores in the micro- and nanometer range. The high permeability and porosity of the silica skeleton and the resulting low back pressure allow for more flexible flow rates compared to particulate columns. As a result, Chromolith® HPLC columns enable high-throughput analysis without loss of separation efficiency or peak capacity.
 

Chromolith® HPLC columns comes in different sizes from capillary to preparative.

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Technical Data

Phase name

Modification

Particle size

Pore size[1]

End-capping

C-content

USP

Surface area

pH range

Tmax

Chromolith® RP-18 e

C18

Monolithic

13 nm / 2 µm

+

18%

L1

300m2/g

2.0-7.5

45 °C

Chromolith® HR RP-18 e

C18

Monolithic

15 nm / 1.15 µm

+

18%

L1

250m2/g

2.0-7.5

45 °C

Chromolith® RP-8 e

C8

Monolithic

13 nm / 2 µm

+

11%

L7

300m2/g

2.0-7.5

45 °C

Chromolith® HR RP-8 e

C8

Monolithic

15 nm / 1.15 µm

+

11%

L7

250m2/g

2.0-7.5

45 °C

Chromolith® Phenyl

Propylphenyl

Monolithic

13 nm / 2 µm

N/A

11%

N/A

300m2/g

2.0-7.5

45 °C

Chromolith® CN

Cyano (CN)

Monolithic

13 nm / 2 µm

N/A

11%

N/A

300m2/g

2.0-7.5

45 °C

Chromolith®NH2

Aminopropyl

Monolithic

13 nm / 2 µm

-

N/A

N/A

300m2/g

2.0-7.5

45 °C

Chromolith® Diol

Diol

Monolithic

13 nm / 2 µm

N/A

11%

N/A

300m2/g

2.0-7.5

45 °C

Chromolith® Si

-

Monolithic

13 nm / 2 µm

N/A

-

L3

300m2/g

2.0-7.5

45 °C

[ 1] For Chromolith® 2 mm i.d. columns tde macropores have an average pore size of 1.5 µm

Chromolith WP 300 columns for bioapplications with 300Å mesopores

Phase name

Modification

Particle size

Pore size[1]

End-capping

C-content

USP

Surface area

pH range

Tmax

Chromolith® WP-300 RP-18

C18

Monolithic

30 nm / 2 µm

N/A

8-9%

N/A

120m2/g

1.5-7.5

45 °C

Chromolith® WP-300 RP-8

C8

Monolithic

30 nm / 2 µm

N/A

4-5%

N/A

120m2/g

1.5-7.5

45 °C

Chromolith® WP-300 RP-4

C4

Monolithic

30 nm / 2 µm

N/A

3-4%

N/A

120m2/g

1.5-7.5

45 °C

Chromolith® WP-300 Protein A

Protein A

Monolithic

30 nm / 2 µm

N/A

N/A

N/A

120m2/g

N/A

N/A

Chromolith® WP-300 Epoxy[1]

Epoxy

Monolithic

30 nm / 2 µm

N/A

5-6%

N/A

120m2/g

N/A

N/A

[ 1 ] For Chromolith® 2 mm i.d. columns the macropores have an average pore size of 1.5 µm

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