ProteoSil HPLC Columns

The latest column series from GL Sciences are the ProteoSil columns. The ProteoSil columns have been specially developed for the requirements of the biopharmaceutical market.

As biopharmaceuticals are much larger than conventional pharmaceuticals, the ProteoSil columns have a pore size of 100, 200 and 300Å, optimised for this analysis. Due to possible interactions of the samples with the hardware, all columns are available with bioinert PEEK lined stainless steel, PEEK or stainless steel to meet all requirements. The small solder to solder differences also make these columns ideal for LC/MS applications.

The ProteoSil columns are available with different modifications and for different chromatographic mechanisms such as reverse phase, HILIC and SEC. Additionally, 1.9µm particles are available for UHPLC applications.

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Technical Data

Phase	Modification	Particle size	Pore size	Molecular weight	Target components
ProteoSil 100-C18	C18	1.9, 3, 5 µm	100 Å	<5,000 Da	Proteins/peptides; oligonucleotides/nucleic acids
ProteoSil 200-C18	C18	1.9, 3, 5 µm	200 Å	5,000-20,000 Da	Proteins/peptides; oligonucleotides/nucleic acids
ProteoSil 300-C18	C18	5 µm	300 Å	>20,000 Da	Proteins/peptides; oligonucleotides/nucleic acids
ProteoSil 200-C8	C8	1.9, 3, 5 µm	200 Å	5,000-20,000 Da	Proteins/peptides; oligonucleotides/nucleic acids
ProteoSil 300-C8	C8	5 µm	300 Å	>20,000 Da	Proteins/peptides; oligonucleotides/nucleic acids
ProteoSil 300-C4	C4	5 µm	300 Å	>20,000 Da	Proteins/Peptides; Monoclonal antibodies; Oligonucleotides/Nucleic acids
ProteoSil HILIC	Amide	1.9, 3, 5 µm	100 Å		Proteins/Peptides; Monoclonal antibodies
ProteoSil 100-SEC	Diol	5 µm	100 Å	<5,000 Da	Proteins/Peptides; Monoclonal antibodies; Oligonucleotides/Nucleic acids
ProteoSil 300-SEC	Diol	5 µm	300 Å	5,000-600,000 Da	Proteins/Peptides; Monoclonal antibodies; Oligonucleotides/Nucleic acids

What characterises the ProteoSil range?

High reproducibility from batch to batch
Carefully designed pore size to fulfil the requirements for biopharmaceutical separations

Bioinert hardware available

When do you benefit from the ProteoSil columns?

You can benefit from ProteoSil columns if a slightly larger pore size than the usual 100 or 120 Å is required. These larger pores allow some analytes to be better separated.

Conclusion: ProteoSil columns are ideal for the biopharmaceutical market. With the different pore sizes of 100, 200 and 300 Å, a wide range of biomolecules can be separated.

ProteoSil

Technical Data

What characterises the ProteoSil range?

When do you benefit from the ProteoSil columns?

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