Agilent Technologies / BioSEC

BioSEC

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Agilent Bio SEC HPLC columns ensure high-resolution and reproducible size-based separations of proteins, peptides and other biomolecules. They are packed with spherical silica particles coated with a proprietary hydrophilic layer, ensuring a highly efficient size exclusion particle. The phases show high stability in high-salt and low-salt conditions and are compatible with most aqueous buffers. Bio SEC columns are available with two different particle sizes and various pore sizes, covering a wide molecular weight range. 

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Technical Data

Phase name

Modification

Particle size

Pore size

USP

pH range

Tmax

Molecular weight range (proteins)

Bio SEC-3 100Å

N/A

3 µm

100 Å

N/A

2.0-8.5

N/A

100 - 100,000 Da

Bio SEC-3 150Å

N/A

3 µm

150 Å

N/A

2.0-8.5

N/A

500 - 150,000 Da

Bio SEC-3 300Å

N/A

3 µm

300 Å

N/A

2.0-8.5

N/A

5,000 - 1,250,000 Da

Bio SEC-5 100Å

N/A

5 µm

100 Å

N/A

2.0-8.5

N/A

100 - 100,000 Da

Bio SEC-5 150Å

N/A

5 µm

150 Å

N/A

2.0-8.5

N/A

500 - 150,000 Da

Bio SEC-5 300Å

N/A

5 µm

300 Å

N/A

2.0-8.5

N/A

5,000 - 1,250,000 Da

Bio SEC-5 500Å

N/A

5 µm

500 Å

N/A

2.0-8.5

N/A

15,000 - 2,000,000 Da

Bio SEC-5 1000Å

N/A

5 µm

1000 Å

N/A

2.0-8.5

N/A

50,000 - 7,500,000 Da

Bio SEC-5 2000Å

N/A

5 µm

2000 Å

N/A

2.0-8.5

N/A

> 10,000,000 Da

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