Sepax / Carbomix

Carbomix

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Carbomix Säulen bestehen aus einem nicht porösen Polystyrol Divinylbenzol Polymer (PS/DVB). Dieses Polymer besitzt Sulfonsäure Gruppen mit verschiedenen chelatisierenden Metallionen auf seiner Oberfläche. Diese Säulen wurden speziell für hochauflösende Trennungen von wasserlöslichen organischen Komponenten wie Kohlenhydrate, organische Säuren, Peptide und kleine bioorganische Moleküle entwickelt.

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Technische Daten

Phase

Gegenion

Partikelgröße

Quervernetzung

pH Bereich

Tmax

Carbomix H-NP

H+

5, 10 μm

5, 8, 10 %

1.0-3.0

85 °C

Carbomix Ca-NP

Ca2+

5, 10 μm

5, 8, 10 %

5.0-9.0

85 °C

Carbomix Pb-NP

Pb2+

5, 10 μm

5, 8, 10 %

5.0-9.0

85 °C

Carbomix K-NP

K+

5, 10 μm

5, 8, 10 %

5.0-9.0

85 °C

Carbomix Na-NP

Na+

5, 10 μm

5, 8, 10 %

5.0-9.0

85 °C

Applikationen

Retentionszeittabelle für Carbomix-Phasen

Verbindung

Carbomix H-NP5 8%

Carbomix H-NP10 5%

Carbomix H-NP10 8%

Carbomix H-NP10 10%

Carbomix Ca-NP5 8%

Carbomix Ca-NP10 5%

Carbomix Ca-NP10 8%

Carbomix Ca-NP10%

Carbomix Pb-NP5 8%

Carbomix Pb-NP10 5%

Carbomix Pb-NP10 8%

Acetic acid

14.11

17.94

15.33

13.70

/

/

/

/

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Adonitol

10.39

13.73

11.76

9.97

14.706

16.7625

14.654

13.67

19.84

21.93

20.07

Arabinitol

/

/

/

/

/

/

/

16.06

/

/

/

D-(-)-Arabinose

10.36

13.76

11.71

9.89

13.6045

15.5905

13.3615

12.52

16.03

17.61

16.08

L-(+)-Arabinose

10.35

13.76

11.71

9.89

13.552

15.5945

13.379

12.53

16.02

17.58

16.12

1,4-Butanediol

19.72

23.35

20.63

19.57

15.428

17.9025

15.412

14.48

16.67

17.66

16.64

n-Butanol

33.74

35.76

33.79

34.38

24.583

27.14

25.19

/

27.93

28.34

28.09

t-Butanol

23.21

26.46

23.8

23.43

16.2755

18.6255

16.243

/

16.97

17.71

16.95

sec-butyl alcohol

28.61

31.29

28.81

28.89

19.8715

22.497

20.095

19.01

21.26

22.35

21.37

D-(+)-Cellobiose

8.34

9.87

9.55

7.87

9.92

10.355

9.055

8.96

11.72

11.92

11.47

Citric acid

8.6

10.6

9.9

8.17

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/

/

/

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Erythriyol

11.27

14.77

12.62

10.86

15.368

17.3185

15.35

14.47

19.62

21.26

19.77

Ethanol

20.66

22.82

21.22

20.63

16.515

18.1795

16.394

15.89

17.08

 

17.09

Formic acid

13.08

16.46

14.3

12.67

/

/

/

/

/

/

/

D-Fructose

9.81

12.9

11.17

9.36

13.5535

15.4645

13.347

12.45

17.06

18.82

17.22

Fumaric acid

12.39

17.07

13.77

11.79

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/

/

/

/

/

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Galactitol

10.07

13.31

11.45

9.64

19.624

22.5455

20.343

18.05

31.46

35.88

32.43

D-(+)-Galactose

9.7

12.77

11.05

9.25

12.1835

13.963

11.809

11.2

14.85

16.35

14.88

D-Glucose

9.36

12

10.64

8.87

11.274

12.554

10.714

10.32

13.29

14.04

13.18

Glycerol

12.68

16.26

13.98

12.29

15.9965

17.8425

15.974

15.12

19.34

20.59

19.42

IPA

22.29

24.87

22.87

22.38

16.434

18.447

16.374

15.88

17.16

17.72

17.11

Lactic acid

11.93

15.69

13.27

11.51

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/

/

/

/

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B-Lactose

8.44

10.41

9.71

8.02

10.221

11.092

9.452

9.22

12.35

13.34

12.21

D-Lyxose

10.07

13.25

11.43

9.61

14.02775

16.167

13.922

13.02

16.5

18.04

16.61

Maleic acid

8.75

9.32

9.96

8.39

/

/

/

/

/

/

/

Malic acid

9.59

12.52

10.93

9.13

/

/

/

/

/

/

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Maltotetraose

/

/

/

/

/

/

/

/

/

/

/

Maltitol

8.55

10.72

9.87

8.14

12.4125

15.0435

12.274

11.24

18.16

22.19

18.65

D-(+)-Maltose

8.37

10.15

9.65

7.94

10.064

10.665

9.268

9.09

12.19

12.8

12.01

Maltotriose

/

/

/

/

/

/

/

8.57

/

/

/

D-Mannitol

9.95

13.14

11.34

9.52

16.92

19.563

17.2445

15.6

24.23

27.79

24.86

D-(+)-Mannose

9.68

12.68

11.02

9.22

12.422

14.238

12.093

11.45

16.23

18.04

16.37

D-(+)-Melezitose

8.05

9.03

9.26

7.63

9.493

9.404

8.51

8.49

10.92

10.9

10.6

Methanol

18.45

20.61

19.14

18.36

16.325

17.7215

16.13

15.64

16.79

17.16

16.69

Oxalic acid dihydrate

7.95

7.53

9.12

7.63

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/

/

/

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1-Propanol

25.24

27.48

25.63

25.57

19.332

21.2605

19.403

18.95

20.75

21.33

20.76

1,2-Propylene glycol

15.89

19.45

17

15.6

17.307

18.8945

17.274

/

20.45

20.97

20.45

Pyruvic acid

/

/

/

/

/

/

/

/

/

/

/

D-(-)-Ribose

10.6

14.04

11.9

10.08

20.842

24.489

21.913

19.23

30.21

34.62

31.1

D-Sorbito

10.05

13.3

11.43

9.61

20.632

23.786

21.2095

18.71

33.51

39.07

34.72

Succinic acid

11.07

15.33

12.49

10.57

/

/

/

/

/

/

/

D-(+)-Sucrose

/

/

/

/

9.9875

10.594

9.175

9.03

11.74

12.12

11.51

Tartartic

8.93

11.06

10.23

8.49

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/

/

/

/

/

/

Triethylene glycol

/

/

/

/

/

/

/

17.21

/

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/

Xylitol

10.75

14.27

12.15

10.33

20.0725

23.067

20.79

18.66

30.7

35.15

31.54

D-Xylose

9.75

12.74

11.11

9.31

12.1335

13.6325

11.692

11.19

14.17

14.94

14.1

Rhamnose

10.09

13.41

11.46

9.64

12.4475

14.172

12.088

 

14.75

15.76

14.79

Trehalose

8.37

10.14

9.64

7.95

9.9815

10.5605

9.158

 

11.8

12.25

11.58

Xylulose

/

/

/

/

 

15.6985

 

 

/

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Propanoic acid

16.04

20.68

17.27

15.65

 

 

 

 

/

/

/

n-butyric acid

18.87

24.7

20.17

18.51

 

 

 

 

/

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/

Isobutyric acid

17.52

23.21

18.83

17.14

 

 

 

 

/

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Butane tetracarboxylic acid

8.43

10.6

9.73

8

 

 

 

 

/

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Sodium acetate

14.11

17.95

15.34

13.7

 

 

 

 

/

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/

Sodium nitrite

/

/

/

/

 

 

 

 

/

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Sodium Pyrosulfite

12.37

12.07

12.93

/

 

 

 

 

/

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Testbedingungen

 

Flussrate: 0.6 mL/min

 

Detektor: RID

 

Injektionsvolumen:  10 µL

 

Säule Mobile Phase Säulen Temperatur
Carbomix H-NP10 2.5mM H2SO4 80 °C
Carbomix H-NP5 2.5mM H2SO4 55 °C
Carbomix Ca-NP10 Wasser 85 °C
Carbomix Ca-NP5 Wasser 85 °C
Carbomix Pb-NP10 Wasser 75 °C
Carbomix Pb-NP5 Wasser 75 °C

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