Brownlee™ ist Markenname für HPLC Säulen des Herstellers Perkin Elmer. Die Phasen basieren auf hochreinem, spärischen Silika-Gel und sind in vielen Modifikationen für unterschiedlichste Anwendungen erhältlich.
Phase-Name | Modifizierung | Partikelgröße | Porengröße | End-capping | C-Gehalt | USP | Oberfläche | pH-Bereich | Tmax |
C18 | C18 | 3, 5 µm | 110 Å | + | 13% | L1 | N/A | 2.5‒7.5 | 80 °C |
C8 | C8 | 5 µm | 110 Å | + | 7% | L7 | N/A | 2.5‒7.5 | 80 °C |
Phenyl | Phenyl | 5 µm | 110 Å | + | 6% | L11 | N/A | 2.5‒7.5 | 80 °C |
Biphenyl | Biphenyl | 5 µm | 110 Å | + | Proprietary | L11 | N/A | 2.5‒7.5 | 80 °C |
Cyano | Cyano (CN) | 5 µm | 110 Å | + | 4% | L10 | N/A | 2.5‒7.5 | 80 °C |
Amino | Amino (NH2) | 3, 5 µm | 110 Å | - | 2% | L8 | N/A | 2.5‒7.5 | 80 °C |
PAH | C18 | 4 µm | 110 Å | + | Proprietary | N/A | N/A | 2.5‒7.5 | 80 °C |
Silica | - | 5 µm | 110 Å | - | - | L3 | N/A | 2.5‒7.5 | 80 °C |
Phase-Name | Modifizierung | Partikelgröße | Porengröße | End-capping | C-Gehalt | USP | Oberfläche | pH-Bereich | Tmax |
C18 | C18 | 1.9, 3, 5 µm | 140 Å | + | 11% | L1 | N/A | 2.5‒8.0 | 80 °C |
Aq C18 | C18 | 1.9, 3, 5 µm | 140 Å | Proprietary | 6% | L1 | N/A | 2.5‒8.0 | 80 °C |
Biphenyl | Biphenyl | 1.9, 3, 5 µm | 140 Å | + | 8% | L11 | N/A | 2.5‒8.0 | 80 °C |
Cyano | Cyano (CN) | 1.9, 5 µm | 140 Å | - | 4% | L10 | N/A | 2.5‒8.0 | 80 °C |
PFP Propyl | Pentafluorophenyl | 1.9, 3, 5 µm | 140 Å | + | 6% | L43 | N/A | 2.5‒8.0 | 80 °C |
IBD | Polar group embedded Alkyl | 1.9 µm | 140 Å | + | Proprietary | L68 | N/A | 2.5‒8.0 | 80 °C |
PAH | C18 | 1.9 µm | 140 Å | + | Proprietary | N/A | N/A | 2.5‒8.0 | 80 °C |
Silica | - | 1.9 µm | 140 Å | - | - | L3 | N/A | 2.5‒8.0 | 80 °C |
Phase-Name | Modifizierung | Partikelgröße | Porengröße | End-capping | C-Gehalt | USP | Oberfläche | pH-Bereich | Tmax |
AP OD-300 (C18) | C18 | 7 µm | 300 Å | + | 10% | L1 | N/A | 2.5‒8.0 | 60 °C |
AP RP-300 (C8) | C8 | 7 µm | 300 Å | + | 5% | L7 | N/A | 2.5‒8.0 | 60 °C |
AP AX-300[1] | Polyetdyleneimine | 7 µm | 300 Å | N/A | N/A | N/A | N/A | 2.0‒8.0 | 60 °C |
AP CX-300[2] | Polyamide coating witd Carboxymetdyl groups | 7 µm | 300 Å | N/A | N/A | N/A | N/A | 2.0‒8.0 | 60 °C |
Bio C18 | C18 | 5 µm | 300 Å | + | 9% | L1 | N/A | 2.5‒8.0 | 80 °C |
Bio C8 | C8 | 5 µm | 300 Å | + | 5% | L7 | N/A | 2.5‒8.0 | 80 °C |
[1] Weak Anion Exchanger [2] Weak Cation Exchanger
Phase-Name | Modifizierung | Partikelgröße | Porengröße | End-capping | C-Gehalt | USP | Oberfläche | pH-Bereich | Tmax |
C18 | C18 | 5 µm | 60 Å | + | 27% | L1 | N/A | 2.5‒8.0 | 80 °C |
Aq C18 | C18 | 5 µm | 60 Å | - | Proprietary | L1 | N/A | 2.5‒8.0 | 80 °C |
Biphenyl | Biphenyl | 5 µm | 60 Å | - | 23% | L11 | N/A | 2.5‒8.0 | 80 °C |
PFP Propyl | Pentafluorophenyl | 3, 5 µm | 60 Å | + | 17% | L43 | N/A | 2.5‒8.0 | 80 °C |
Basix | Phenyl Cyano | 5 µm | 60 Å | + | 12% | L10 | N/A | 2.5‒8.0 | 80 °C |
Organic Acid[1] | N/A | 5 µm | 60 Å | + | Proprietary | N/A | N/A | 2.5‒8.0 | 80 °C |
AK[2] | N/A | 5 µm | 60 Å | - | Proprietary | N/A | N/A | 2.5‒8.0 | 80 °C |
[1] Special phase for the separation of organic acids
[2] Special phase for the separation of DNPH derivatized Aldehydes and Ketones
Phase-Name | Modifizierung | Partikelgröße | Porengröße | End-capping | C-Gehalt | USP | Oberfläche | pH-Bereich | Tmax |
Spheri-5 C18 | C18 | 5 µm | 80 Å | + | 11% | L1 | N/A | N/A | N/A |
Spheri-5 C18 ODS (Polyfunctional) | C18 | 5 µm | 80 Å | + | 14% | L1 | N/A | N/A | N/A |
Spheri-10 C18 | C18 | 10 µm | 80 Å | + | 11% | L1 | N/A | N/A | N/A |
Spheri-10 C2 | C2 | 10 µm | 80 Å | - | N/A | L16 | N/A | N/A | N/A |
Spheri-5 Silica | - | 5 µm | 80 Å | N/A | N/A | L3 | N/A | N/A | N/A |
Phase-Name | Modifizierung | Partikelgröße | Porengröße | End-capping | C-Gehalt | USP | Oberfläche | pH-Bereich | Tmax |
C18 | C18 | 3, 5 µm | 100 Å | + | 20% | L1 | N/A | 2.5‒8.0 | 80 °C |
Aq C18 | C18 | 3, 5 µm | 100 Å | - | 15% | L1 | N/A | 2.5‒8.0 | 80 °C |
C8 | C8 | 5 µm | 100 Å | + | 12% | L7 | N/A | 2.5‒8.0 | 80 °C |
C4 | C4 | 5 µm | 100 Å | + | 9% | L26 | N/A | 2.5‒8.0 | 80 °C |
C1 | C1 | 5 µm | 100 Å | - | 5% | L13 | N/A | 2.5‒8.0 | 80 °C |
Phenyl | Phenyl | 3, 5 µm | 100 Å | + | 10% | L11 | N/A | 2.5‒8.0 | 80 °C |
Cyano | Cyano (CN) | 5 µm | 100 Å | + | 8% | L10 | N/A | 2.5‒8.0 | 80 °C |
Amino | Amino (NH2) | 3, 5 µm | 100 Å | - | 2% | L8 | N/A | 2.5‒8.0 | 80 °C |
PFP Propyl | Pentafluorophenyl | 5 µm | 100 Å | + | 11% | L43 | N/A | 2.5‒8.0 | 80 °C |
IBD | Polar group embedded Alkyl | 3, 5 µm | 100 Å | - | 12% | L68 | N/A | 2.5‒8.0 | 80 °C |
Carbamate[1] | N/A | 3, 5 µm | 100 Å | Proprietary | Proprietary | N/A | N/A | 2.5‒8.0 | 80 °C |
Quat[2] | N/A | 5 µm | 100 Å | Proprietary | Proprietary | N/A | N/A | 2.5‒8.0 | 80 °C |
[1] Special phase for the analysis of diquat, paraquat and other quaternary amines
[2] Special phase for the analysis of carbamates
Phase-Name | Modifizierung | Partikelgröße | Porengröße | End-capping | C-Gehalt | USP | Oberfläche | pH-Bereich | Tmax |
C18 | C18 | 2.7 µm | 90 Å | + | N/A | N/A | N/A | 2.0‒9.0 | 60 °C |
Peptide ES-C18 | C18 | 2.7 µm | 160 Å | + | N/A | N/A | N/A | 1.0‒8.0 | 90 °C |
C8 | C8 | 2.7 µm | 90 Å | + | N/A | N/A | N/A | 2.0‒9.0 | 60 °C |
PhenylHexyl | Phenylhexyl | 2.7 µm | 90 Å | + | N/A | N/A | N/A | 2.0‒9.0 | 60 °C |
PFP | Pentafluorophenyl | 2.7 µm | 90 Å | + | N/A | N/A | N/A | 2.0‒8.0 | 60 °C |
RP-Amide | Amide embedded Alkyl | 2.7 µm | 90 Å | + | N/A | N/A | N/A | 2.0‒9.0 | 60 °C |
HILIC | N/A | 2.7 µm | 90 Å | - | N/A | N/A | N/A | 2.0‒8.0 | 60 °C |