Mixed-Mode Phasen für die HPLC nutzen verschiedene Trenntechniken in einer Säule parallel. Die stationären Phasen sind dafür speziell/verschieden modifiziert, sodass z.B. gleichzeitig Reversed-Phase und Ionenaustauscher-Eigenschaften an der Trennung mitwirken. Heutzutage gibt es viele verschiedene Mixed-Mode Phasen die die unterschiedlichsten Trennmodi miteinander kombinieren. Sie zeigen einzigartige Selektivitäten, sodass sie für manch schwierige Trennung die Säule der Wahl darstellen.
| Hersteller | Phase-Name | Modifizierung | Beteiligte Trennmechanismen | Partikelgröße | Porengröße | pH-Bereich |
| Imtakt | Scherzo SS-C18 | C18 + SAX + SCX | Reversed-Phase Anion Exchange Cation Exchange Normal Phase | 3 µm | 130 Å | N/A |
| Scherzo SM-C18 | C18 + WAX + WCX | Reversed-Phase Anion Exchange Cation Exchange Normal Phase | 3 µm | 130 Å | N/A | |
| Scherzo SW-C18 | C18 + SAX + SCX | Reversed-Phase Anion Exchange Cation Exchange Normal Phase | 3 µm | 130 Å | N/A | |
| Shodex | Asahipak GS-HQ[1] | N/A | Size Exclusion (SEC) Reversed Phase HILIC | 6, 7 & 13 µm | 150, 400, | 2.0‒9.0 |
| Sepax | HP-SCX | Sulfonic Acid + Phenyl | Cation Exchange Reversed Phase | 1.8, 3, 5 & 10 µm | 120 Å | 1.5‒8.0 |
| HP-SAX | Quat. Ammonium + Phenyl | Anion Exchange Reversed Phase | 5 µm | 120 Å | 1.5‒8.0 | |
| Osaka Soda | Capcell Pak CR | C18 + SCX | Reversed Phase Cation Exchange | 3 & 5 µm | N/A | N/A |
| Thermo Scientific | Acclaim Mixed-Mode HILIC-1 | Alkyl chain with | Reversed-Phase HILIC | 3 & 5 µm | 120 Å | 2.5‒7.5 |
| Acclaim Mixed-Mode WAX-1 | Alkyl chain with | Reversed Phase Anion Exchange | 3 & 5 µm | 120 Å | 2.5‒7.5 | |
| Acclaim Mixed-Mode WCX-1 | Alkyl chain with | Reversed Phase Cation Exchange Anion Exclusion | 3 & 5 µm | 120 Å | 2.5‒7.5 | |
| Acclaim Trinity P1 | RP + SCX + WAX | Reversed Phase Anion Exchange Cation Exchange | 3 µm | 300 Å | 2.5‒7.0 | |
| Acclaim Trinity P2 | WCX + SAX | HILIC Anion Exchange Cation Exchange | 3 µm | 300 Å | 2.0‒8.0 | |
| Acclaim Trinity Q1 | RP + WCX + WAX | Reversed Phase Anion Exchange Cation Exchange | 3 µm | 300 Å | 2.5‒7.5 | |
| Sielc | Primesep A | RP + embedded SCX | Reversed Phase Cation Exchange | 5 & 10 µm | 100 Å | N/A |
| Primesep 100 | RP + embedded CX | Reversed Phase Cation Exchange | 2.7, 3, 5, & 10 µm | 100 Å | N/A | |
| Primesep 200 | RP + embedded WCX | Reversed Phase Cation Exchange | 5 & 10 µm | 100 Å | N/A | |
| Primesep B | RP + embedded SAX | Reversed Phase Anion Exchange | 5 & 10 µm | 100 Å | N/A | |
| Primesep B2 | RP + embedded WAX | Reversed Phase Anion Exchange | 5 & 10 µm | 100 Å | N/A | |
| Primesep AB | RP + embedded AX + CX | Reversed-Phase Anion Exchange Cation Exchange | 5 & 10 µm | 100 Å | N/A | |
| Primesep C | Proprietary | Reversed Phase Cation Exchange Complex Formation | 5 & 10 µm | 100 Å | N/A |
[1] Base Material: Polyvinylalcohol